A promising method for preparing metaphase spread for counting the number of chromosomes from the emerging shoot tissue is described in this report. In the present study. we adopted enzymatic digestion of shoot tips to analyse the chromosome number. The chromosomes in metaphase stage of cell division are highly condensed and easy to count in routine cytological technique. https://www.parisnaturalfoodes.shop/product-category/all-in-one-lightly-sweet/